- Description Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ 10B chemically competent E. coli cells provide higher transformation efficiency than any competitor’s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. Our DirectPlate™ 10B chemically competent cells have the highest transformation efficiency on the market. Please find a video below displaying the convenience and capability of this product. Specifications Competent cell type: Chemically competent Derivative of: DH10B Species: E. coli Format: Tubes Transformation efficiency: ≥1 x 108 – 109 cfu/µg pUC19 DNA Blue/white screening: Yes Shipping condition: Dry ice Reagents Needed for One Reaction DirectPlate™ 10B competent cell: 50 µl DNA (or pUC19 Control, 10 pg/µl): 1 µl Storage DirectPlate™ 10B competent cells: -80 ºC pUC19 control DNA: -20 ºC Genotype F – mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) nupG λ- Quality Control Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×108 – 109 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity. General Guidelines Follow these guidelines when using DirectPlate™ 10B chemically competent E. coli. Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting. Thaw competent cells on ice and transform cells immediately following thawing. After adding DNA, mix by gently pipetting up and down a few times. 1013-13 1013-36 Comparison Testing Intact Genomics has performed extensive comparison testing to prove our DirectPlate™ 10B chemically competent cells have the highest transformation efficiency on the market.1015-12 1015-36
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