- Description IgScript™ Probe-Based One Step RT-qPCR 2x master mix contains IgScript™ Reverse Transcriptase, Taq DNA polymerase, MgCl2, dNTPs, stabilizers and low ROX reference dye with standard buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ Probe-Based One Step RT-qPCR Kit is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in RT-qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions. IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification. Probe-Based One Step RT-qPCR Kit Includes: IgScript one step RT-qPCR 2x master mix Applications Gene expression data validation. Multiplexing Mutation detection Pathogen and viral detection Genetically modified organisms (GMO) characterization and Genetic profiling Benefits Enhanced efficiency, specificity, and sensitivity Compatible with all real-time PCR instruments Superior gene expression results under various cycling conditions Robust and active for cDNA synthesis at temperatures up to 55°C. Storage Temperature –20 °C4212 4215 4217
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