- Description T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits (1) is the product of a recombinant gene in E. coli . Mutations generated by CRISPR/ZFN/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. If the mismatched DNA length position is more than 1 bp, T7 Endonuclease I can recognize and cleave it. It is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells. Applications Resolve four-way junction or branched DNA Detect or cleave heteroduplex and nicked DNA Randomly cleave linear DNA for shot-gun cloning Protein purity The physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below). Product Source E coli BL21 (DE3) pLysS strain expressing T7 Endonuclease I gene. Product Includes T7 Endonuclease I 10x T7 Endonuclease I reaction buffer 1x T7 Endonuclease I reaction buffer 10 mM Tris-HCL, 50 mM KCl ,10 mM MgCl2 , 1 mM DTT, pH 7.5 @ 25ºC Storage Buffer 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC Storage Temperature –20ºC Quality Control assays This product is free from detectable contaminating nuclease activities. 3610 3612 Technical Support Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.
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