- Description Intact Genomics is your resource for simple and fast cleanup of RNAs including sgRNAs. This kit purifies and concentrates up to 50 µg of RNA from enzymatic reactions. We recommend using this kit following sgRNA synthesis via the IG® sgRNA Synthesis Kits to remove proteins, salts and most unincorporated nucleotides. The Intact Genomics RNA Cleanup Kit has a simplified workflow, as shown below, and is ideal for cleanup and concentration of RNA after enzymatic treatments such as DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT). Workflow Benefits The Intact Genomics IG® RNA Cleanup Kit is a perfect choice for simple and fast cleanup of up to 50 µg of RNA. Below is a sampling of the key benefits. Rapid and simplified workflow with a single wash buffer Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt) Option to Elute in 20-50 µL for more concentrated RNA than the standard 100 µL Standard 70-100% RNA recovery Customizable buffer options for difficult reactions Purified RNA is ready for use in a wide variety of downstream applications including: RT-PCR, RNA library prep for NGS, formation of RNP complexes for genome editing, microinjection, RNA labeling, and transfection. Components and Storage The IG® RNA Cleanup Kit contains the items below. Store all components at Room Temperature. RNA Cleanup Columns (50 μg capacity) Collection Tubes RNA Cleanup Binding Buffer RNA Cleanup Wash Buffer (concentrated) Nuclease-free Water Comparative Data We compared Intact Genomics’ IG® RNA Cleanup Kit with New England Biolabs’ Monarch® RNA Cleanup Kit (50 μg) with 7 RNA samples (50 μg each) and 50 μl of elution buffer. The differences in the eluted amounts are not statistically significant. 4003 4005
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