IG® Autoinduction Medium

$ 76.50

Category: Accessories

Description Intact Genomics (IG®) Autoinduction Medium promotes high-yield growth of E. coli and high-level expression of T7 promoter-regulated recombinant proteins without time-consuming steps such as monitoring optical density (OD) or adding induction components like IPTG. Unlike traditional LB-IPTG induction systems, IG® Autoinduction Medium allows regulated protein expression in any expression system that is solely inducible by IPTG from E. coli strains containing a fully functional lac operon. We have also developed an Autoinduction DE3 strain. Both chemical (catalog # 1065) and electroporation (Catalog # 1265) competent cells are available. These cells have been used to test a broad variety of protein targets resulting in robust protein expression and production (Figure 1). Protein expression begins automatically after inoculation with a single colony or starter culture. After an incubation time of ~7 hours, the culture is shifted to a lower temperature overnight for continued growth and protein expression under standard conditions. Figure 1. Lane 1: The proteins were expressed in IG® autoinduction medium Lane 2: The proteins were expressed in LB with IPTG Lane 3: Protein molecular weight marker Four examples of the genes of different recombinant proteins that were cloned into a common pET30b vector and were used to transform IG® AutoInduction DE3 competent cells. Each expression culture (100mL of cells) was collected, cells were lysed, and whole cell extracts were analyzed on a Coomassie Blue stained SDS-PAGE gel with ~ 11% Bis- buffer system. Autoinduction lanes (1) show more robust protein expression (~3-fold to 10-fold) higher in these examples. Key Benefits: Simple & Fast: No need for frequent OD600 monitoring or manually adding an inducer—freeing your time to focus on other tasks. Repeatable: Reliable, consistent protein expression by reducing: leaky expression, vector instability, and challenges with toxic proteins. High-Yield: Achieve high cell density and robust protein expression with ease. Flexibility: Compatible with other DE3 strains and media for seamless integration into your workflow. Product Components: Component 1 (in one of two optional formats): 1-L: liquid, 1000 mL, ready-to-use (includes Component 2) 1-P: powder, 40 grams Component 2: 50mL Package Formats: Component 1-L: bottle; Component 1-P: bag; Component 2: bottle Shipping Condition: Room Temperature Storage: 4⁰C Vectors and Bacterial Strains IG® Autoinduction Medum is designed for inducible T7 promoter-regulated protein expression systems. IG® Autoinduction DE3 strain is optimized and shows a higher success rate using IG® Autoinduction Medium and simple growth conditions than other types of BL21(DE3) strains below. T7 promoter-regulated expression vectors (such as pET) are suitable for cloning your gene of interest. E. coli expression strains that contain a fully functional lac operon (including lacY and lacZ) such as BL21(DE3), and BL21(DE3)pLysS strains can be used with IG® Autoinduction Medium. Important Tips: Some E.coli expression strains including T7-regulated expression systems with a partially functional lac operon (such as NEB T7 expression strain), and E. coli cloning strains such as DH10B, DH5alpha, etc. CANNOT be used for autoinduction of protein expression. 1722 1722-L 1722-P 1722-1L 1722-5L 1722Pd-1L

IG® Autoinduction Medium

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