- Description Intact Genomics (ig®) Staphylococcus aureus (S. aureus) RN4220 Electrocompetent Cells are optimized to provide high transformation efficiencies making them ideal for various applications. This strain is commonly used in studies involving virulence, resistance, metabolism and more. Staphylococcus aureus RN4220 is characterized by a mutation in the sau1 hsdR genes, making it restriction deficient and hence an excellent intermediate cloning host. Specifications Competent cell type: Electrocompetent Species: S. aureus Strain: RN4220 Format: Tubes Transformation efficiency: ≥1 x 105 cfu/µg DNA Blue/white screening: No Shipping condition: Dry ice Reagents Included ig® Staphylococcus aureus (S. aureus) RN4220 Electrocompetent Cells Recovery medium Storage ig® Staphylococcus aureus (S. aureus) RN4220 Electrocompetent Cells: -80 ºC Recovery medium: 4 ºC Quality Control Transformation efficiency is tested by using the control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be equal or greater than 1×105 CFU/µg DNA. Untransformed cells are tested for appropriate antibiotic sensitivity. General Guidelines Follow these guidelines when using ig® S. aureus Electrocompetent Cells: • Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting. • Thaw competent cells on ice and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing. Biosafety Level: 2 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories. 5th ed. Washington, DC: U.S. Government Printing Office, 2009; see: www.cdc.gov/biosafety/publications/bmbl5/index.htm. BSL2 facility is required for purchase and use of this product. 1294-20 1294-40
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