- Description Intact Genomics (IG®) Autoinduction DE3 ElectroCompetent cells are suitable for transformation and routine recombinant protein expressed from a vector under an IPTG-inducible T7 promoter. This Autoinduction strain with the DE3 lysogen, which contains a chromosomally integrated IPTG-inducible promoter enabling T7 RNA polymerase, is expressed by IPTG induction, as well as autoinduction with IG® optimized Autoinduction media. (catalog #: 1722). With simple and less hands-on autoinduction, you can either enjoy more free-time or get more work done. Moreover, the yields of both expressed cells and protein are likely 5-10 fold or more for a broad range of protein targets at either small or large scale of protein production. Specifications: Competent cell type: Electroporation Competent Strain: IG Autoinduction DE3 cells which contain a chromosomally integrated IPTG-inducible promoter Species: E. coli Format: Tubes Transformation efficiency: ≥ 1.0 x 1010 cfu/µg pUC19 DNA Shipping condition: Dry ice Reagents Needed for One Reaction: IG Autoinduction DE3 ElectroCompetent cells: 25 µl DNA (or pUC19 Control, 10 pg/µl): 1 µl Recovery medium: 1 ml Product Components & Storage: IG Autoinduction DE3 ElectoCompetent cells: -80 ºC pUC19 control DNA: -20 ºC Recovery medium: 4 ºC Genomic Features: IG Autoinduction DE3 chemically competent cells have the following features: T7 Expression Strain Optimized for autoinduction Suitable for expression of toxic genes TetR: Tetracycline resistant (12.5 µg/ml) Quality Control: Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and the high efficiency transformation protocol listed below. Transformation efficiency should be ≥1.0 x 1010 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity. General Guidelines: Follow these guidelines when using IG Autoinduction DE3 ElectroCompetent cells: Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting. Thaw competent cells on ice and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing. Note: A high-voltage electroporation apparatus such as Bio-Rad Gene Pulser II #165-2105, capable of generating field strengths of 16 kV/cm is required. 1265 1265-06 1265-12 1265-24 1265-48
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