- Description Intact Genomics (IG®) C41(DE3) Electrocompetent Cells are designed for transformation and routine recombinant protein expression from vectors under an IPTG-inducible T7 promoter. The C41(DE3) strain is a BL21(DE3) mutant originally selected as survivor cells overexpressing the toxic oxoglutarate-malate carrier protein (OGCP). It carries lacI and additional mutations that help prevent cell death associated with the expression of many toxic recombinant proteins. IG® C41(DE3) Electrocompetent Cells enable efficient expression of toxic proteins from a wide range of organisms, including viruses, bacteria, yeasts, plants, animals, and mammals. The effectiveness of the C41(DE3) strain for expressing toxic proteins has been demonstrated in a patent and validated in more than 400 publications. Specifications: Competent cell type: Electro competent Species: E. coli Strain: C41(DE3) Format: Tubes Transformation efficiency: ≥ 1.0 x 1010 cfu/µg pUC19 DNA Blue/white screening: No Shipping condition: Dry ice Reagents Needed for One Reaction IG® C41(DE3) Electrocompetent cells: 25 µl DNA (or pUC19 Control, 10 pg/µl): 1 µl Recovery medium: 1 ml Product Components and Recommended Storage Condition: IG® C41(DE3) Electrocompetent Cells: -80°C DNA (pUC19, 10 pg/µl): -20°C Recovery medium: 4°C Genomic Features: IG® C41(DE3) Electrocompetent Cells have the following features: T7 Expression Strain Selected for expression of toxic proteins Suitable for expression of toxic genes Quality Control: Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and the high efficiency transformation protocol listed below. Transformation efficiency should be ≥ 1.0 x 1010 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity. General Guidelines: Follow these guidelines when using IG C41(DE3) Electrocompetent Cells. Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting. Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing. 1243 1243-06 1243-24
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