- Description Intact Genomics (IG®) C41(DE3)pLysS Electrocompetent Cells are designed for transformation and routine recombinant protein expressed from a vector driven by an IPTG-inducible T7 promoter. The C41(DE3) strain is a derivative of BL21(DE3) , was selected as survivor cells over-expressing the toxic oxoglutarate-malate carrier protein (OGCP), has lacI and other mutations, which prevent cell death associated with expression of many other recombinant toxic proteins. As a result, the C41(DE3)pLysS Electrocompetent Cells is highly effective in expressing toxic proteins from all organisms, including viruses, bacteria, yeasts, animals, plants, and mammals. In addition, C41(DE3)pLysS strain also carries a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. This strain is used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins. The effectiveness of C41(DE3)pLysS strain for expressing toxic proteins has been validated in >400 publications including all mutations were unveiled in genome sequencing. Specifications: Competent cell type: Electro competent Species: E. coli Strain: C41(DE3)pLysS Format: Tubes Transformation efficiency:≥ 1.0 x 109 cfu/µg pUC19 DNA Blue/white screening: No Shipping condition: Dry ice Reagents Needed for One Reaction IG® C41(DE3)pLysS Electrocompetent cells: 25 µl DNA (or pUC19 Control, 10 pg/µl): 1 µl Recovery medium: 1 ml Product Components and Recommended Storage Condition: IG® C41pLysS(DE3) Electrocompetent Cells: -80°C DNA (pUC19, 10 pg/µl): -20°C Recovery medium: 4°C Genomic Features: IG® C41(DE3)pLysS Electrocompetent Cells have the following features: T7 Expression Strain Selected for expression of toxic proteins Suitable for expression of toxic genes Quality Control: Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and the high efficiency transformation protocol listed below. Transformation efficiency should be ≥ 1.0 x 109CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity. General Guidelines: Follow these guidelines when using IG C41(DE3)pLysS Electrocompetent Cells. Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting. Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing. 1245 1245-12 1245-48
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