- Description Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity (1). Note: Glycerol acts as a cryoprotectant and protein stabilizer when added to the storage buffer of proteins and enzymes. However, in certain circumstances, it is preferred to omit glycerol from the buffer. This includes instances where the presence of glycerol may interfere, such as lyophilization, high-throughput instruments with sensitive fluidics or primary cell cultures. For our glycerol free products, it is recommended to use immediately upon thaw as without glycerol there will be significant activity loss with freeze/thaw cycles. Applications • Strand displacement DNA synthesis (2) • Random primer labeling • Second strand cDNA synthesis • dA-tailing Protein Purity The physical purity of Bsu DNA Polymerase I, Large Fragment is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below). Product Source E. coli strain expressing Bsu Polymerase I gene lacking the N-terminal exonuclease domain. Product Includes • Glycerol Free Bsu DNA Polymerase I, Large fragment • 10x Bsu DNA Polymerase I reaction buffer 1x Bsu Polymerase I reaction buffer 10 mM Tris-HCl 50 mM KCl 10 mM MgCl2 1 mM DTT pH 7.9 @ 25°C Storage Buffer 50 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA, pH 7.5 @ 25ºC Storage Temperature –20ºC Heat inactivation 70°C for 20 min Unit Definition One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 37º C. Quality Control assays Glycerol Free Bsu Polymerase I, Large fragment is free from detectable nuclease activities. Quality control is performed following the production of each new lot of products to ensure that it meets the quality standards and specifications designated for the product. References 1. Okazaki, T. et al. (1964) J. Biol. Chem. 239, 259–268. 2. Piepenburg, O. et al. (2006) PLOS Biology, 4, 1115–1121.3582G 3585G3582GF 3585GF 3587GF 3582Lyo 3585Lyo 3587Lyo
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